Preliminary Phytochemical Screening and Antibacterial Activity of Datura Metel and Vitex Negundo against Bacterial Cold Water Disease Causing Organism

Objective: Effect of medicinal plants on pathogens and disease causing organism are believedto possess good activity against it. The medicinal plant Vitex negundo and Datura metel were studie for analyzing antibacterial effect against Bacterial Cold Water Disease causing organism. Methods: The selected plant leaves were extracted out with following solvent of Hexane Chloroform and Aqueous and tested against Flavobacterium psychrophilum,Paper Disc diffusion method was followed with varying concentration of 50-250 mg/ml and then these plants were subjected to preliminary phytochemical analysis by following standard protocol of Harbone’s phytochemical analysis. Results: The phytochemical analysis resulted in presence of terpenoids, phytosterols, steroids, flavonid and phenol compounds. Some of the secondary metabolites that could have acted against Flavobacterium psychrophilum for its sensitivity against plant extracts, pathogen showed anti bacterial activity of 15mm sensitivity at 250 mg/ml concentration for both plant extracts and sensitivity were seen from 150mg/ml concentration. Conclusion: The medicinal value rich plants could be used in treating or preventing Bacterial Cold Water disease or infection with higher specification of its bioactive compound for the development of chemical constituent or drug molecules.